Volume 7 Supplement 6

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

Meeting abstracts

Edited by Hansjörg Hauser

Publication charges for this supplement were funded by the European Society for Animal Cell Technology (ESACT). The Supplement Editor declares that he has no competing interests.

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health.

Lille, France23-26 June 2013

Concatenated PDF 7S6

Page 2 of 3

Size-dependent antioxidative activity of platinum nanoparticles

Authors: Hidekazu Nakanishi, Takeki Hamasaki, Tomoya Kinjo, Kiichiro Teruya, Shigeru Kabayama and Sanetaka Shirahata

Citation: BMC Proceedings 2013 7(Suppl 6):P41

Content type: Poster presentation Published on: 4 December 2013
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Sampling and quenching of CHO suspension cells for the analysis of intracellular metabolites

Authors: Judith Wahrheit and Elmar Heinzle

Citation: BMC Proceedings 2013 7(Suppl 6):P42

Content type: Poster presentation Published on: 4 December 2013
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¹³C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells

Authors: Judith Wahrheit, Averina Nicolae and Elmar Heinzle

Citation: BMC Proceedings 2013 7(Suppl 6):P43

Content type: Poster presentation Published on: 4 December 2013
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Investigation of glutamine metabolism in CHO cells by dynamic metabolic flux analysis

Authors: Judith Wahrheit, Averina Nicolae and Elmar Heinzle

Citation: BMC Proceedings 2013 7(Suppl 6):P44

Content type: Poster presentation Published on: 4 December 2013
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The optimization of a rapid low-cost alternative of large-scale medium sterilization

Authors: Dominique T Monteil, Cédric A Bürki, Lucia Baldi, David L Hacker, Maria de Jesus and Florian M Wurm

Citation: BMC Proceedings 2013 7(Suppl 6):P45

Content type: Poster presentation Published on: 4 December 2013
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Improved fed-batch bioprocesses using chemically modified amino acids in concentrated feeds

Authors: Ronja Mueller, Isabell Joy-Hillesheim, Karima El Bagdadi, Maria Wehsling, Christian Jasper, Joerg von Hagen and Aline Zimmer

Citation: BMC Proceedings 2013 7(Suppl 6):P46

Content type: Poster presentation Published on: 4 December 2013
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Approaches for automized expansion and differentiation of human MSC in specialized bioreactors

Authors: Anne Neumann, Antonina Lavrentieva, Dominik Egger, Tim Hatlapatka and Cornelia Kasper

Citation: BMC Proceedings 2013 7(Suppl 6):P47

Content type: Poster presentation Published on: 4 December 2013
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Cell cycle and apoptosis in PER.C6^® cultures

Authors: Sarah M Mercier, Bas Diepenbroek, Dirk E Martens, Rene H Wijffels and Mathieu Streefland

Citation: BMC Proceedings 2013 7(Suppl 6):P48

Content type: Poster presentation Published on: 4 December 2013
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Scale-up considerations for monoclonal antibody production process: an oxygen transfer flux approach

Authors: Laura Gimenez, Claire Simonet and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P49

Content type: Poster presentation Published on: 4 December 2013
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Improvement of production rate on recombinant CHO cells in two-stage culture

Authors: Hiroshi Matsuoka, Chie Shimizu and Mihoko Tazawa

Citation: BMC Proceedings 2013 7(Suppl 6):P50

Content type: Poster presentation Published on: 4 December 2013
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HEK293 cell culture media study: increasing cell density for different bioprocess applications

Authors: Leticia Liste-Calleja, Martí Lecina and Jordi Joan Cairó

Citation: BMC Proceedings 2013 7(Suppl 6):P51

Content type: Poster presentation Published on: 4 December 2013
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Preliminary studies of cell culture strategies for bioprocess development based on HEK293 cells

Authors: Leticia Liste-Calleja, Jonatan López-Repullo, Martí Lecina and Jordi Joan Cairó

Citation: BMC Proceedings 2013 7(Suppl 6):P52

Content type: Poster presentation Published on: 4 December 2013
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Adhesion and colonization of mesenchymal stem cells on polylactide or PLCL fibers dedicated for tissue engineering

Authors: Frédérique Balandras, Caroline Ferrari, Eric Olmos, Mukesh Gupta, Cécile Nouvel, Jérôme Babin, Jean-Luc Six, Nguyen Tran, Isabelle Chevalot, Emmanuel Guedon and Annie Marc

Citation: BMC Proceedings 2013 7(Suppl 6):P53

Content type: Poster presentation Published on: 4 December 2013
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Cell cycle and apoptosis: a map for the GS-NS0 cell line at the genetic level and the link to environmental stress

Authors: Chonlatep Usaku, David Garcia Munzer, Efstratios N Pistikopoulos and Athanasios Mantalaris

Citation: BMC Proceedings 2013 7(Suppl 6):P54

Content type: Poster presentation Published on: 4 December 2013
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Design space definition for a stirred single-use bioreactor family from 50 to 2000 L scale

Authors: Thomas Dreher, Ute Husemann, Sebastian Ruhl and Gerhard Greller

Citation: BMC Proceedings 2013 7(Suppl 6):P55

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Full transcriptome analysis of Chinese Hamster Ovary cell lines producing a dynamic range of Coagulation Factor VIII

Authors: Christian S Kaas, Claus Kristensen, Jens J Hansen, Gert Bolt and Mikael R Andersen

Citation: BMC Proceedings 2013 7(Suppl 6):P56

Content type: Poster presentation Published on: 4 December 2013
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Production of monoclonal antibody, Anti-CD3 by hybridoma cells cultivated in Basket Spinner under free and immobilized conditions

Authors: Elsayed A Elsayed, Hoda Omar, Hasnaa R Shahin, Hamida Abou-Shleib, Maha El-Demellawy, Mohammad Wadaan and Hesham A El-Enshasy

Citation: BMC Proceedings 2013 7(Suppl 6):P57

Content type: Poster presentation Published on: 4 December 2013
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Using Rice Bran Extract (RBE) as Supplement for Mescenchymal Stem Cells (MSCs) in Serum-free Culture

Authors: Rinaka Yamauchi, Ken Fukumoto, Satoko Moriyama, Masayuki Taniguchi, Shigeru Moriyama, Takuo Tsuno and Satoshi Terada

Citation: BMC Proceedings 2013 7(Suppl 6):P58

Content type: Poster presentation Published on: 4 December 2013
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Viral vector production in the integrity^® iCELLis^® single-use fixed-bed bioreactor, from bench-scale to industrial scale

Authors: Alexandre Lennaertz, Shane Knowles, Jean-Christophe Drugmand and Jose Castillo

Citation: BMC Proceedings 2013 7(Suppl 6):P59

Content type: Poster presentation Published on: 4 December 2013
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Linear scalability of virus production in the integrity^® iCELLis^® single-use fixed-bed bioreactors from bench to industrial scale

Authors: Shane Knowles, Jean-Christophe Drugmand, Nicolas Vertommen and Jose Castillo

Citation: BMC Proceedings 2013 7(Suppl 6):P60

Content type: Poster presentation Published on: 4 December 2013
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Scale-up of hepatic progenitor cells from multitray stack to 2-D bioreactors

Authors: Matthieu Egloff, Florence Collignon, Jean-François Michiels, Jonathan Goffinet, Sarah Snykers, Philippe Willemsen, Christophe Gumy, Claude Dedry, Jose Castillo and Jean-Christophe Drugmand

Citation: BMC Proceedings 2013 7(Suppl 6):P61

Content type: Poster presentation Published on: 4 December 2013
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Characterization and quantitation of fluorescent Gag virus-like particles

Authors: Sonia Gutiérrez-Granados, Laura Cervera, Francesc Gòdia and María Mercedes Segura

Citation: BMC Proceedings 2013 7(Suppl 6):P62

Content type: Poster presentation Published on: 4 December 2013
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BI-HEX^®-GlymaxX^® cells enable efficient production of next generation biomolecules with enhanced ADCC activity

Authors: Anja Puklowski, Till Wenger, Simone Schatz, Jennifer Koenitzer, Jochen Schaub, Barbara Enenkel, Anurag Khetan, Hitto Kaufmann and Anne B Tolstrup

Citation: BMC Proceedings 2013 7(Suppl 6):P63

Content type: Poster presentation Published on: 4 December 2013
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Effects of perfusion processes under limiting conditions on different Chinese Hamster Ovary cells

Authors: Anica Lohmeier, Tobias Thüte, Stefan Northoff, Jeff Hou, Trent Munro and Thomas Noll

Citation: BMC Proceedings 2013 7(Suppl 6):P64

Content type: Poster presentation Published on: 4 December 2013
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Development of 3D human intestinal equivalents for substance testing in microliter-scale on a multi-organ-chip

Authors: Annika Jaenicke, Dominique Tordy, Florian Groeber, Jan Hansmann, Sarah Nietzer, Carolin Tripp, Heike Walles, Roland Lauster and Uwe Marx

Citation: BMC Proceedings 2013 7(Suppl 6):P65

Content type: Poster presentation Published on: 4 December 2013
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A robust RMCE system based on a CHO-DG44 platform enables efficient evaluation of complex biological drug candidates

Authors: Thomas Rose, Annette Knabe, Rita Berthold, Kristin Höwing, Anne Furthmann, Karsten Winkler and Volker Sandig

Citation: BMC Proceedings 2013 7(Suppl 6):P66

Content type: Poster presentation Published on: 4 December 2013
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Systems biology of unfolded protein response in recombinant CHO cells

Authors: Kamal Prashad Segar, Vikas Chandrawanshi and Sarika Mehra

Citation: BMC Proceedings 2013 7(Suppl 6):P67

Content type: Poster presentation Published on: 4 December 2013
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Chemical chaperone suppresses the antibody aggregation in CHO cell culture

Authors: Masayoshi Onitsuka, Miki Tatsuzawa, Masahiro Noda and Takeshi Omasa

Citation: BMC Proceedings 2013 7(Suppl 6):P68

Content type: Poster presentation Published on: 4 December 2013
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Dynamical analysis of antibody aggregation in the CHO cell culture with Thermo Responsive Protein A (TRPA) column

Authors: Masahiro Noda, Masayoshi Onitsuka, Miki Tatsuzawa, Ichiro Koguma and Takeshi Omasa

Citation: BMC Proceedings 2013 7(Suppl 6):P69

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Fucoidan extract enhances the anti-cancer activity of chemotherapeutic agents in breast cancer cells

Authors: Sanetaka Shirahata, Zhonguan Zhang, Toshihiro Yoshida, Hiroshi Eto and Kiichiro Teruya

Citation: BMC Proceedings 2013 7(Suppl 6):P70

Content type: Poster presentation Published on: 4 December 2013
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Assessment of troglitazone induced liver toxicity in a dynamically perfused two-organ Micro-Bioreactor system

Authors: Eva-Maria Materne, Caroline Frädrich, Reyk Horland, Silke Hoffmann, Sven Brincker, Alexandra Lorenz, Mathias Busek, Frank Sonntag, Udo Klotzbach, Roland Lauster, Uwe Marx and Ilka Wagner

Citation: BMC Proceedings 2013 7(Suppl 6):P71

Content type: Poster presentation Published on: 4 December 2013
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Dynamic culture of human liver equivalents inside a micro-bioreactor for long-term substance testing

Authors: Eva-Maria Materne, Ilka Wagner, Caroline Frädrich, Ute Süßbier, Reyk Horland, Silke Hoffmann, Sven Brincker, Alexandra Lorenz, Matthias Gruchow, Frank Sonntag, Udo Klotzbach, Roland Lauster and Uwe Marx

Citation: BMC Proceedings 2013 7(Suppl 6):P72

Content type: Poster presentation Published on: 4 December 2013
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Evaluation of the advanced micro-scale bioreactor (ambr™) as a highthroughput tool for cell culture process development

Authors: Frédéric Delouvroy, Guillaume Le Reverend, Boris Fessler, Gregory Mathy, Mareike Harmsen, Nadine Kochanowski and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P73

Content type: Poster presentation Published on: 4 December 2013
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Optimized fermentation conditions for improved antibody yield in hybridoma cells

Authors: Martina Stützle, Alina Moll, René Handrick and Katharina Schindowski

Citation: BMC Proceedings 2013 7(Suppl 6):P74

Content type: Poster presentation Published on: 4 December 2013
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High performance CHO cell line development platform for enhanced production of recombinant proteins including difficult-to-express proteins

Authors: Pierre-Alain Girod, Valérie Le Fourn, David Calabrese, Alexandre Regamey, Deborah Ley and Nicolas Mermod

Citation: BMC Proceedings 2013 7(Suppl 6):P75

Content type: Poster presentation Published on: 4 December 2013
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Enhancement mechanism of antioxidant enzyme gene expression by hydrogen molecules

Authors: Tomoya Kinjo, Takeki Hamasaki, Hanxu Yan, Hidekazu Nakanishi, Tomohiro Yamakawa, Kiichiro Teruya, Shigeru Kabayama and Sanetaka Shirahata

Citation: BMC Proceedings 2013 7(Suppl 6):P76

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Evaluation of the impact of matrix stiffness on encapsulated HepaRG spheroids

Authors: Sofia P Rebelo, Marta Estrada, Rita Costa, Christophe Chesné, Catarina Brito and Paula M Alves

Citation: BMC Proceedings 2013 7(Suppl 6):P77

Content type: Poster presentation Published on: 4 December 2013
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Feeding strategy optimization in interaction with target seeding density of a fed-batch process for monoclonal antibody production

Authors: Marie-Françoise Clincke, Grégory Mathy, Laura Gimenez, Guillaume Le Révérend, Boris Fessler, Jimmy Stofferis, Bassem Ben Yahia, Nicola Bonsu-Dartnall and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P78

Content type: Poster presentation Published on: 4 December 2013
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Process development and optimization of fed-batch production processes for therapeutic proteins by CHO cells

Authors: Marie-Françoise Clincke, Mareike Harmsen and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P79

Content type: Poster presentation Published on: 4 December 2013
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Characterization of mAb aggregates in a mammalian cell culture production process

Authors: Albert Paul and Friedemann Hesse

Citation: BMC Proceedings 2013 7(Suppl 6):P80

Content type: Poster presentation Published on: 4 December 2013
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Identification of process relevant miRNA in CHO cell lines - Process profiling reveals interesting targets for cell line engineering

Authors: Fabian Stiefel, Matthias Hackl, Johannes Grilliari and Friedemann Hesse

Citation: BMC Proceedings 2013 7(Suppl 6):P81

Content type: Poster presentation Published on: 4 December 2013
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Introducing a new chemically defined medium and feed for hybridoma cell lines

Authors: Christoph Heinrich, Tim F Beckmann, Sandra Klausing, Stefanie Maimann, Bernd Schröder and Stefan Northoff

Citation: BMC Proceedings 2013 7(Suppl 6):P82

Content type: Poster presentation Published on: 4 December 2013
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Comparative study of bluetongue virus serotype 8 production on BHK-21 cells in a 50L Biostat^® STR single-use bioreactors vs roller bottles

Authors: Lídia Garcia, Mercedes Mouriño and Alicia Urniza

Citation: BMC Proceedings 2013 7(Suppl 6):P83

Content type: Poster presentation Published on: 4 December 2013
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Golgi engineering of CHO cells by targeted integration of glycosyltransferases leads to the expression of novel Asn-linked oligosaccharide structures at secretory glycoproteins

Authors: Tobias Reinl, Nicolas Grammel, Sebastian Kandzia, Eckart Grabenhorst and Harald S Conradt

Citation: BMC Proceedings 2013 7(Suppl 6):P84

Content type: Poster presentation Published on: 4 December 2013
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Characterization of the influence of cultivation parameters on extracellular modifications of antibodies during fermentation

Authors: Christian Hakemeyer, Martin Pech, Gero Lipok and Alexander Herrmann

Citation: BMC Proceedings 2013 7(Suppl 6):P85

Content type: Poster presentation Published on: 4 December 2013
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Technology transfer and scale down model development strategy for biotherapeutics produced in mammalian cells

Authors: Nadine Kochanowski and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P86

Content type: Poster presentation Published on: 4 December 2013
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A modular flow-chamber bioreactor concept as a tool for continuous 2D- and 3D-cell culture

Authors: Christiane Goepfert, Grit Blume, Rebecca Faschian, Stefanie Meyer, Cedric Schirmer, Wiebke Müller-Wichards, Jörg Müller, Janine Fischer, Frank Feyerabend and Ralf Pörtner

Citation: BMC Proceedings 2013 7(Suppl 6):P87

Content type: Poster presentation Published on: 4 December 2013
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Platform process will give platform product - Can we afford it?

Authors: Rohit Diwakar, Sunaina Prabhu, Lavanya C Rao, Janani Kanakaraj, Kriti Shukla, Saravanan Desan, Dinesh Baskar, Ankur Bhatnagar and Anuj Goel

Citation: BMC Proceedings 2013 7(Suppl 6):P88

Content type: Poster presentation Published on: 4 December 2013
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Applications of biomass probe in PAT

Authors: Chandrashekhar K Nanjegowda, Nirmala K Ramappa, Pradeep V Ravichandran, Deepak Vengovan, Saravanan Desan, Dinesh Baskar, Ankur Bhatnagar and Anuj Goel

Citation: BMC Proceedings 2013 7(Suppl 6):P89

Content type: Poster presentation Published on: 4 December 2013
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Understanding cell behavior in cultivation processes - A metabolic approach

Authors: Jonas Aretz, Tobias Thüte, Sebastian Scholz, Klaudia Kersting, Thomas Noll and Heino Büntemeyer

Citation: BMC Proceedings 2013 7(Suppl 6):P90

Content type: Poster presentation Published on: 4 December 2013
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23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

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