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Implementation of a quality by design approach into cell line and process development for recombinant protein production

Authors: Caroline Noack, Anne Schüler and Markus Wieland

Citation: BMC Proceedings 2015 9(Suppl 9):P79

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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A method to determine and simulate the permeation through a gel matrix in a multi-organ-chip

Authors: Hao-Hsiang Hsu, Laura E Harder, Gerd Lindner, Uwe Marx and Ralf Pörtner

Citation: BMC Proceedings 2015 9(Suppl 9):P77

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Phase I clinical trials of safety and immunogenicity of live cultural influenza vaccine vector-flu

Authors: Elena A Nechaeva, Marina P Bogryantseva, Alexander B Ryzhikov, Ol'ga G P'yankova, Tatyana Y Sen'kina, Irina F Radaeva, Sergey Y Nechaev, Natalya I Ryndyuk, Vladimir I Kuzubov, Zoya I Gin'ko, Irina V Kiseleva and Larisa G Rudenko

Citation: BMC Proceedings 2015 9(Suppl 9):P73

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Evaluation of the capability of a real-time PCR assay to detect mycoplasma in advanced therapy medicinal products

Authors: Alexandra Scholz, Stephanie Schweizer and Dirk Vollenbroich

Citation: BMC Proceedings 2015 9(Suppl 9):P72

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Generation of novel microcarrier for expansion of human mesenchymal stem cells

Authors: Dave Splan, Grishma Patel, Heather Woolls and Mark Szczypka

Citation: BMC Proceedings 2015 9(Suppl 9):P71

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Serum-free media for mesenchymal stem cells expansion on microcarriers

Authors: Céline Martin, Alexandre Piccini, Isabelle Chevalot, Eric Olmos, Emmanuel Guedon and Annie Marc

Citation: BMC Proceedings 2015 9(Suppl 9):P70

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Intracellular trafficking pathways for plasmid DNA complexed with highly efficient endosome escape polymers

Authors: Marianne Gillard, Zhongfan Jia, Jeff Hou, Michael Song, Peter P Gray, Trent P Munro and Michael J Monteiro

Citation: BMC Proceedings 2015 9(Suppl 9):P69

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Dynamic cultivation of human stem cells under physiological conditions

Authors: Dominik Egger, Markus Krammer, Jan Hansmann, Heike Walles and Cornelia Kasper

Citation: BMC Proceedings 2015 9(Suppl 9):P68

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Ex vivo production of red blood cells from human cord blood

Authors: Marta Caminal, Juan P Labrozzi, Irene Oliver-Vila, Martí Alzaga-Gragera, Silvia Marín-Gallén, Arnau Pla, Joan García and Joaquim Vives

Citation: BMC Proceedings 2015 9(Suppl 9):P67

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Development of a tool box for mesenchymal stem cell secretome analysis

Authors: Cornelia Kasper, Anna Koch, Verena Charwat and Martina Marchetti-Deschmann

Citation: BMC Proceedings 2015 9(Suppl 9):P66

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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In-line mammalian cell concentration measurement with a Bio Cell Vitality Analyzer

Authors: Patrick Steger and Dirk Lütkemeyer

Citation: BMC Proceedings 2015 9(Suppl 9):P64

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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PAIA assays: A new bead-based assay system for high throughput protein quantification

Authors: Christine I Wosnitza, Kristina Lechner and Sebastian Giehring

Citation: BMC Proceedings 2015 9(Suppl 9):P62

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Development of a Novel Thermoplastic Tubing, FP-FLEX™, and Single-Use Freezing Bag for Working Cell Banks Enabling Closed-System Processing to Temperatures as Low as -196°C

Authors: Dominic Clarke, Doug Norris and Bradley Bennett

Citation: BMC Proceedings 2015 9(Suppl 9):P58

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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A Simplified implementation of the O.U.R. stationary liquid mass balance estimation method for On-line monitoring in Animal cell production processes

Authors: Andreu Fontova, Jonatan López-Repullo, Martí Lecina, Ramon Bragós and Jordi J Cairó

Citation: BMC Proceedings 2015 9(Suppl 9):P56

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Manufacturing ultra-concentrated liquid feeds: Transitioning the aqueous solubility barrier of the feed amino acids cysteine and tyrosine

Authors: Claudia Knack, Caroline Zessel, Christian Schultheiß, Doris Matheis, Christian Schild, Jörg von Hagen, Michael Rayner and Jochen Sieck

Citation: BMC Proceedings 2015 9(Suppl 9):P54

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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CHO-DHFR cell line development platform: Application of Clonepix and Automated Mini Bioreactor (AMBR) technologies to meet accelerated timelines

Authors: Venkata R Mangalampalli, Dyane Wycuff, Mingzhong Chen, David Berlinger, Elizabeth H Scheideman, Amritha Menon, Guilia Fabozzi, Althaf Hussain and Richard M Schwartz

Citation: BMC Proceedings 2015 9(Suppl 9):P52

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Synthetic peptide matrices as support for stem cells culture

Authors: Youlia Serikova, Martin Bousmanne, Jean-Christophe Drugmand, Marc Fouassier, Laurent Jeannin and Yves-Jacques Schneider

Citation: BMC Proceedings 2015 9(Suppl 9):P50

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Real-time monitoring of the physiological state of CHO cells in bioreactor by using a dual spectroscopic strategy

Authors: Franck Courtès, Bruno Ebel, Emmanuel Guedon and Annie Marc

Citation: BMC Proceedings 2015 9(Suppl 9):P48

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Recent developments in scaling down and using single use probes for measuring the live cell concentration by dielectric spectroscopy

Authors: John Carvell, Matthew Lee and Aditya R Bhat

Citation: BMC Proceedings 2015 9(Suppl 9):P46

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Model-based strategy for cell culture seed train layout verified at lab scale

Authors: Simon Kern, Oscar B Platas, Martin Schaletzky, Volker Sandig, Björn Frahm and Ralf Pörtner

Citation: BMC Proceedings 2015 9(Suppl 9):P44

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Model-based DoE for feed batch cultivation of a CHO cell line

Authors: Johannes Möllerl, Regine Eibl, Dieter Eibl and Ralf Pörtner

Citation: BMC Proceedings 2015 9(Suppl 9):P42

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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High-titer expression of recombinant antibodies by transiently transfected HEK 293-6E cell cultures

Authors: Volker Jäger, Johanna Groenewold, Dominik Krüger, Dennis Schwarz and Veith Vollmer

Citation: BMC Proceedings 2015 9(Suppl 9):P40

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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The potential of small molecules to modulate glycosylation by media design

Authors: David Brühlmann, Anaïs Muhr, Jürgen Hemberger, Markus Sauer, Henri Kornmann, Martin Jordan and Hervé Broly

Citation: BMC Proceedings 2015 9(Suppl 9):P38

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44

Authors: David Reinhart, Lukas Damjanovic, Wolfgang Sommeregger, Andreas Gili, Stanislaus Schafellner, Andreas Castan, Christian Kaisermayer and Renate Kunert

Citation: BMC Proceedings 2015 9(Suppl 9):P36

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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From Clone Selection to 80L Bioreactor Production: Better, Faster, Leaner

Authors: Marie-Françoise Clincke, Frédéric Delouvroy, Guillaume L Révérend, Jimmy Stofferis, Matthew Hinchliffe and Laetitia Malphettes

Citation: BMC Proceedings 2015 9(Suppl 9):P34

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Identification of performance drivers for an antibody producing CHO-S cell line culture in the Allegro™ XRS 20 single-use bioreactor utilizing historical data

Authors: Todd Sanderson, Alex Martino, Ing-Kae Wang, Amanda Rose, Byron Rees and Lisa Bradbury

Citation: BMC Proceedings 2015 9(Suppl 9):P33

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Monitorization of pH and OTR using a multiple shake flask platform: A tool for metabolism and cell growth assessment in mammalian cell cultures

Authors: Leticia Liste-Calleja, Tibor Anderlei, Jonatan López-Repullo, Adrián Urbano, Martí Lecina and Jordi J Cairó

Citation: BMC Proceedings 2015 9(Suppl 9):P32

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Spectroscopic tools for an automated suspension cell culture screening system

Authors: Carsten Musmann, Klaus Joeris and Sven Markert

Citation: BMC Proceedings 2015 9(Suppl 9):P31

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Quality compliance in the development of cell-based medicines in non-pharma environments

Authors: Irene Oliver-Vila, Amy L van Deusen, Roger Palau and Joaquim Vives

Citation: BMC Proceedings 2015 9(Suppl 9):P29

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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An improved model framework linking the extracellular environment to antibody glycosylation

Authors: Philip M Jedrzejewski, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2015 9(Suppl 9):P28

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Medium development beyond production media: Chemically defined media for transfection and single cell cultivation

Authors: Tim F Beckmann, Sandra Klausing, Sebastian Püngel, Juliana Coronel, Tim Welsink and Christoph Heinrich

Citation: BMC Proceedings 2015 9(Suppl 9):P27

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Novel small scale TFF cell retention device for perfusion cell culture systems

Authors: Chris S Martin, Jorge Padilla-Zamudio, Doug Rank, Patrick McInnis, Mikhail Kozlov, Sarah Reynolds, Joe Parella and Lee Madrid

Citation: BMC Proceedings 2015 9(Suppl 9):P25

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Optimization and scale-up of cell culture and purification processes for production of an adenovirus-based tuberculosis vaccine

Authors: Chun F Shen, Danielle Jacob, Zhongqi Shao, Alice Bernier, Xuefeng Yu, Mehul Patel, Tao Zhu and Amine Kamen

Citation: BMC Proceedings 2015 9(Suppl 9):P23

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

Authors: Nadine Kochanowski, Gaetan Siriez, Larissa Mukankurayija, Aurélie Delangle, Alex Murray-Smith, Kevin Dromer, David Mainwaring, Clemens Stilling, Nadja Prang, Laetitia Malphettes and Annick Gervais

Citation: BMC Proceedings 2015 9(Suppl 9):P21

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Technology toolbox for cell line development - next generation cell line development technologies

Authors: Holger Laux, Ursula Bodendorf, Sandrine Romand, Anett Ritter, Thomas Jostock and Burkhard Wilms

Citation: BMC Proceedings 2015 9(Suppl 9):P17

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Purification of modified vaccinia virus Ankara from suspension cell culture

Authors: Ingo Jordan, Diana Weimer, Stefan Iarusso, Holger Bernhardt, Verena Lohr and Volker Sandig

Citation: BMC Proceedings 2015 9(Suppl 9):O13

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Generation and characterization of a superior host cell line for biomanufacturing

Authors: Anett Ritter, Johannes Wienberg, Bernd Voedisch, Burkhard Wilms, Sabine Geisse, Thomas Jostock and Holger Laux

Citation: BMC Proceedings 2015 9(Suppl 9):P13

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Protein aerosol for intranasal nose to brain (N2B) delivery

Authors: Martina Stützle, Stefan Carle, Lucas Engelhardt, Ulrich Simon, Annette Schafmeister, Chrystelle Mavoungou and Katharina Schindowski

Citation: BMC Proceedings 2015 9(Suppl 9):O11

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion

Authors: YuanShan Lai, Noriko Yamano, Masayoshi Onitsuka and Takeshi Omasa

Citation: BMC Proceedings 2015 9(Suppl 9):P11

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Cellular signaling pathways modulated by influenza infection in HEK293 cells

Authors: Aziza P Manceur, Amine A Kamen, Emma Petiot, Chun F Shen and Sven Ansorge

Citation: BMC Proceedings 2015 9(Suppl 9):P9

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Development of an advanced cell therapy product indicated for the treatment of gonarthrosis

Authors: Joaquim Vives, Margarita Blanco, Marta Caminal, Maria I Coca, Margarita Codinach, Ruth Coll, Manuel Doral, Mireia Lloret, Irene Oliver-Vila, Isabel Ortega, Laura Reales, Míriam Requena-Montero, Luciano Rodríguez, Sílvia Torrents and Joan García

Citation: BMC Proceedings 2015 9(Suppl 9):O9

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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RNAi-mediated knockdown of Dnmt3a enhances antibody titer in CHO cells up to 200 percent

Authors: Anica Schmidt, Anna Wippermann, Raimund Hoffrogge and Thomas Noll

Citation: BMC Proceedings 2015 9(Suppl 9):P8

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Identification of bottlenecks in antibody expression using targeted gene integration

Authors: Patrick Mayrhofer, Alexander Mader, Bernhard Kratzer, David Reinhart, Willibald Steinfellner, Wolfgang Sommeregger and Renate Kunert

Citation: BMC Proceedings 2015 9(Suppl 9):P7

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Process parameters impacting product quality

Authors: Jan Bechmann, Frederik Rudolph, Lucy Gebert, Jochen Schaub, Benedikt Greulich, Michael Dieterle and Harald Bradl

Citation: BMC Proceedings 2015 9(Suppl 9):O7

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Cre-mediated cellular modification for establishing producer CHO cells of recombinant scFv-Fc

Authors: Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Akira Ito and Masamichi Kamihira

Citation: BMC Proceedings 2015 9(Suppl 9):P5

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Non-fucose level as a function of glycoenzyme transcription in a glycoengineered CHO cell line

Authors: Sven Markert, Harald Wizemann and Christine Jung

Citation: BMC Proceedings 2015 9(Suppl 9):O5

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Establishment and characterization of new human cell lines for recombinant therapeutic protein production

Authors: Yuichi Inoue, Akira Iwamoto, Aiko Inoue and Hiroharu Kawahara

Citation: BMC Proceedings 2015 9(Suppl 9):P3

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Production of IgGs with a human-like sialylation in CHO cells

Authors: Céline Raymond, Anna Robotham, Maureen Spearman, Michael Butler, John Kelly and Yves Durocher

Citation: BMC Proceedings 2015 9(Suppl 9):O3

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Stability difference of each chromosome in Chinese Hamster Ovary cell line

Authors: Noriko Yamano, Toshitaka Kumamoto, Mai Takahashi, Jana Frank, Masayoshi Onitsuka and Takeshi Omasa

Citation: BMC Proceedings 2015 9(Suppl 9):P1

Content type: Poster presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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Front-line of genome editing technology for animal cell engineering

Authors: Tetsushi Sakuma

Citation: BMC Proceedings 2015 9(Suppl 9):O1

Content type: Oral presentation Published on: 14 December 2015

This article is part of a Supplement: Volume 9 Supplement 9
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