Fig. 1 (abstract P-061).

The standard transfection process (A) and modified processes (B&C) were evaluated in shake flask cultures (a & b) and SUB and RBr scale (c & d). Process B was noted to increase cell growth (b) whilst process C reduced cell growth and resulted in a slight titre drop (a). At SUB scale Process C reduced cell growth to levels comparable to the RBr culture, an effect that was scalable from 50L to 250L (d) and produced titres that were comparable to the RBr cultures (c)